human lamp2  (Developmental Studies Hybridoma Bank)

Journal: Biomolecules

Article Title: Cholesterol Modulation Attenuates the AD-like Phenotype Induced by Herpes Simplex Virus Type 1 Infection

doi: 10.3390/biom14050603

Figure Lengend Snippet: Summary of antibodies employed in Western blot (WB) and immunofluorescence (IF) experiments.

Article Snippet: , Human LAMP2 , Mouse , 1/1000 (WB) 1/50 (IF) , DSHB H4B4.

Techniques: Western Blot, Immunofluorescence, Infection, Plasmid Preparation

Journal: Biomolecules

Article Title: Cholesterol Modulation Attenuates the AD-like Phenotype Induced by Herpes Simplex Virus Type 1 Infection

doi: 10.3390/biom14050603

Figure Lengend Snippet: HSV-1 infection induces intracellular cholesterol accumulation. ( A ) Intracellular cholesterol levels expressed as nanograms of cholesterol per micrograms of protein in SK-N-MC and N2a neuroblastoma cells treated with U18666A and water-soluble cholesterol. ( B ) Intracellular levels of cholesterol in cultures infected with HSV-1 at a multiplicity of infection (MOI) of 10 for 18 h compared to mock-infected cells. The graph data show the mean ± SEM of at least 4 independent experiments. Significance was recorded at p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***). ( C ) Confocal microscopy images of filipin staining (green) show cholesterol distribution patterns in SK-N-MC and N2a cells infected with HSV-1 (MOI 10) or treated with U18666A. TO-PRO-3-stained nuclei (red) are also shown. Immunofluorescence images of SK-N-MC cells uninfected ( D ) or infected with HSV-1 at MOI 10 ( E ) were obtained with filipin staining (green) and antibodies recognizing different markers (red) of early (EEA1) and late endosomes (CD222), autophagosomes (LC3), and lysosomes (LAMP2). TO-PRO-3-stained nuclei (blue) are also shown. Arrowheads in the merge panels showed the colocalization of filipin with CD222 and LC3 in HSV-1-infected cells. Scale bar: 10 µm.

Article Snippet: , Human LAMP2 , Mouse , 1/1000 (WB) 1/50 (IF) , DSHB H4B4.

Techniques: Infection, Confocal Microscopy, Staining, Immunofluorescence

Journal: Frontiers in Immunology

Article Title: Autoantibodies Against Lysosome Associated Membrane Protein-2 (LAMP-2) in Pediatric Chronic Primary Systemic Vasculitis

doi: 10.3389/fimmu.2020.624758

Figure Lengend Snippet: Concentration of LAMP-2-ANCA in pediatric chronic small-to-medium vessel vasculitis patients. (A) LAMP-2-ANCA concentration (y-axis; ng/mL) in serum of individuals with early-onset vasculitis that are known to be positive (n = 5) and negative (n = 1) for LAMP-2-ANCA (squares) , children with vasculitis (n = 51, circles), and children with systemic autoinflammatory disease (n = 5, triangles). (B) LAMP-2-ANCA concentration (y-axis; ng/mL) in pediatric patients grouped (x-axis) based on positivity for MPO-ANCA (n = 19), PR3-ANCA (n = 23), or neither MPO- or PR3-ANCA (ANCA-, n = 7), and (C, D) LAMP-2-ANCA concentration (y-axis; ng/mL) plotted against (C) MPO-ANCA (x-axis; U/L) (n =19) and (D) PR3-ANCA (x-axis; U/L) (n = 23). Bars show median. Horizontal line divided low (<1,000 ng/mL) and moderate-high positive LAMP-2-ANCA (>1,000 ng/mL). Open symbols on the x-axis denote samples below the lower limit of detection of the assay (n = 4 patients with vasculitis, and n = 1 patient with autoinflammatory disease).

Article Snippet: Standards were generated using anti-human LAMP-2 monoclonal antibody (H4B4) (Invitrogen, CA, USA) serially diluted in BB, with optimal dilution range of 50–1,000 ng/ml.

Techniques: Concentration Assay

Journal: Frontiers in Immunology

Article Title: Autoantibodies Against Lysosome Associated Membrane Protein-2 (LAMP-2) in Pediatric Chronic Primary Systemic Vasculitis

doi: 10.3389/fimmu.2020.624758

Figure Lengend Snippet: Comparison of LAMP-2-ANCA titer with standard clinical measures of disease activity. Concentration of LAMP-2-ANCA (y-axis; ng/mL) in pediatric vasculitis patients plotted against (A) C-reactive protein (CRP) concentration (x-axis; mg/L) (n = 51), (B) erythrocyte sedimentation rate (ESR) (x-axis; mm/h) (n = 44), and (C) pediatric vasculitis activity score (pVAS) (x-axis) (n = 51) at the time of diagnosis, and (D) blood samples taken prior to (naïve, n = 14), or after (treated, n = 37), induction of immune suppressive therapy. Bars show median. Horizontal line divided low (<1,000 ng/mL) and moderate-high positive LAMP-2-ANCA (>1000 ng/mL). Open symbols on the x-axis denote samples below the lower limit of detection of the assay (n = 4).

Article Snippet: Standards were generated using anti-human LAMP-2 monoclonal antibody (H4B4) (Invitrogen, CA, USA) serially diluted in BB, with optimal dilution range of 50–1,000 ng/ml.

Techniques: Activity Assay, Concentration Assay, Sedimentation

Journal: Frontiers in Immunology

Article Title: Autoantibodies Against Lysosome Associated Membrane Protein-2 (LAMP-2) in Pediatric Chronic Primary Systemic Vasculitis

doi: 10.3389/fimmu.2020.624758

Figure Lengend Snippet: Comparison of LAMP-2-ANCA titer with renal metrics. Concentration of LAMP-2-ANCA (y-axis: ng/ml) in pediatric vasculitis patients: (A) with the absence (n = 15) and presence (n = 36) of proteinuria (x-axis) at the time of diagnosis. (B) plotted against change in GFR from the time of diagnosis to 12-month follow-up (x-axis, ml/min/1.73m 2 ) (n = 25, p = 0.0314). (C) with no renal involvement (renal PVAS < 4; n = 5), and either renal improvement (increase in GFR at 12-month > 10 ml/min/1.73m 2 , n = 8) or worsening (decrease in GFR at 12-month > 10 ml/min/1.73m 2 , n = 12) from diagnosis to 12-month follow-up. (D) plotted against serum creatinine concentration (x-axis; µmol/L) (n = 26, p = 0.2149). Bars show median.

Article Snippet: Standards were generated using anti-human LAMP-2 monoclonal antibody (H4B4) (Invitrogen, CA, USA) serially diluted in BB, with optimal dilution range of 50–1,000 ng/ml.

Techniques: Concentration Assay